Enssure Malaria Pv/Pf Assay kit
Malaria infections in humans are primarily caused by four species of Plasmodium parasites, with P. falciparum and P. vivax being the most significant due to their distinct characteristics. P. falciparum is associated with cerebral malaria and drug resistance, while P. vivax is known for its high infectivity and relapse rates. The choice of treatment depends on the specific species, making the differentiation between P. falciparum and P. vivax crucial for effective patient management and recovery.EnSSure Malaria (PV/PF) utilizes distinct detection systems for these two species. It employs the detection of P. falciparum-specific histidine-rich protein-2 (Pf. HRP-2) to identify P. falciparum malaria, as this protein is released from parasitized erythrocytes. For P. vivax malaria, the test relies on the presence of P. vivax-specific pLDH (plasmodium lactate dehydrogenase). This differentiation enables accurate diagnosis and appropriate treatment, ensuring better outcomes for patients.
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Malaria Pv/Pf
Product Information
Intended Use:
Rapid Malaria Pv/Pf (Pv-pLDH /Pf -HRPII) Ag Test is a lateral Flow chromatographic immunoassay for the simultaneous detection and differentiation of Plasmodium vivax (Pv-pLDH) and Plasmodium falciparum (Pf -HRPII) antigen in human blood specimen.
Principle:
The Rapid Malaria Pv/Pf Antigen Test employs a membrane strip with pre-coated test lines for Pv pLDH and Pf HRP2 antigens, along with a control line. Upon introducing a blood sample and assay buffer, the lysed blood reacts with gold-conjugated antibodies, forming complexes. These complexes generate colored bands on respective test lines for Pv pLDH and Pf HRP2, confirming positive results. A control line with goat anti-Rabbit IgG serves as a procedural control, ensuring accurate test performance.
Testing Procedure
Importance to know
Warnings and Precautions
- Please read the instruction carefully before performing the test.
- Do not use the test after the expiration date.
- Immediately carry out the test after removing the test device from the pouch.
- Do not mix or interchange the specimen sample.
Limitation
- The test is limited to the detection of antigen to Malaria Plasmodium sp. Although the test is very accurate in detecting pLDH and HRP-2, a low incidence of false results can occur.
- In case of (Pv+Pf) mixed infections, detects P. vivax as low as 100 parasite/µl even in presence of high P. falciparum densities of ~ 1,00,000 parasite/µl. In suspected cases of P. falciparum densities > 1,00,000 parasite/µl, confirm the results with microscopy.
- In P. falciparum infections, the 'Pf' band may be absent in carriers during gametogony. A positive result after treatment doesn't necessarily indicate treatment failure; persistent positivity after 5-10 days may suggest a resistant strain.
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